Ratiometric Fluorescence Coding for Multiplex Nucleic Acid Amplification Testing
نویسندگان
چکیده
منابع مشابه
IsoPCR: an analytically sensitive, nested, multiplex nucleic acid amplification method.
BACKGROUND Nucleic acid tests that can simultaneously detect multiple targets with high sensitivity, specificity, and speed are highly desirable. To meet this need, we developed a new approach we call the isoPCR method. METHODS The isoPCR method is a 2-stage nested-like nucleic acid amplification method that combines a single multiplex preamplification PCR with subsequent distinct detection o...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2018
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.8b03266